|
KMID : 0545120210310081060
|
|
Journal of Microbiology and Biotechnology
2021 Volume.31 No. 8 p.1060 ~ p.1068
|
|
|
Comparative Study of the Difference in Behavior of the Accessory Gene Regulator (Agr) in USA300 and USA400 Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA)
|
|
Lee Hye-Soo
Song Hun-Suk
Lee Hong-Ju
Kim Sang-Hyun
Suh Min-Ju
Cho Jang-Yeon
Ham Si-On
Kim Yun-Gon
Joo Hwang-Soo
Kim Woo-Seong
Lee Sang-Ho
Yoo Dong-Won
Bhatia Shashi Kant
Yang Yung-Hun
|
|
|
Abstract
|
|
|
|
Community-associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) is notorious as a leading cause of soft tissue infections. Despite several studies on the Agr regulator, the mechanisms of action of Agr on the virulence factors in different strains are still unknown. To reveal the role of Agr in different CA-MRSA, we investigated the LACΔagr mutant and the MW2Δagr mutant by comparing LAC (USA300), MW2 (USA400), and Δagr mutants. The changes of Δagr mutants in sensitivity to oxacillin and several virulence factors such as biofilm formation, pigmentation, motility, and membrane properties were monitored. LACΔagr and MW2Δagr mutants showed different oxacillin sensitivity and biofilm formation compared to the LAC and MW2 strains. Regardless of the strain, the motility was reduced in Δagr mutants. And there was an increase in the long chain fatty acid in phospholipid fatty acid composition of Δagr mutants. Other properties such as biofilm formation, pigmentation, motility, and membrane properties were different in both Δagr mutants. The Agr regulator may have a common role like the control of motility and straindependent roles such as antibiotic resistance, biofilm formation, change of membrane, and pigment production. It does not seem easy to control all MRSA by targeting the Agr regulator only as it showed strain-dependent behaviors.
|
|
|
KEYWORD
|
|
|
CA-MRSA, USA300, USA400, function of Agr system, strain-dependent behaviors
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|